Biogazelle's tech notes

Development of Bio-Rad's PrimePCR assays and arrays for lncRNA expression analysis

J. Hellemans

Bio-Rad has expanded its PrimePCR portfolio of real-time PCR gene expression assays to include Biogazelle's assays and array plates for human long non-coding RNA. lncRNAs play important regulatory roles in both normal and disease biology, but they also present certain challenges for qPCR analysis. Here we discuss the methodology used for the design of PrimePCR Assays and Arrays.

Comparison of mRNA and lncRNA expression

Comprehensive RNA seq data quality control and reliable data processing using Cobra

Comprehensive RNA seq data quality control and reliable data processing using Cobra

Guide to preparation of liquid biopsies for nucleic acid extraction

J. Vandesompele

A successful experiment starts with a good experiment design. Based on our extensive experience in analysis of nucleic acids, we share some recommendations for your liquid biopsy experiment. We discuss experiment design, collection and preservation of liquid biopsy samples, and nucleic acid extraction.

Getting the most out of your samples – microRNA enrichment by depleting abundant undesirable small RNAs in biofluids

small RNA sequencing results of a typical mouse serum sample after tRNA fragment removal

Total RNA sequencing of formalin-fixed paraffin-embedded samples detects long non-coding RNAs

Highly sensitive and reproducible detection of genes

Unlocking the transcriptome in formalin-fixed paraffin embedded tissue using mRNA capture sequencing

Small RNA sequencing of RNA isolated from body fluids

Highly reproducible and sensitive small RNA sequencing workflow

Small RNA sequencing of RNA isolated from FFPE tissues

Detection of differentially expressed miRNAs in colon cancer versus control samples

TruSeq total RNA sequencing performance assessment