RNA sequencing allows for unbiased, specific and sensitive quantification of coding and non-coding RNA transcripts. In addition to classic RNA expression analysis, Biogazelle also exploits the structural information encoded in the transcriptome, such as splice variants, gene fusions, mutations, and RNA editing events. RNA sequencing is the preferred method for gene signature discovery, for the molecular characterization of tumors, to unveil disease mechanisms or discern patient subgroups, to gain a clear understanding of a drug mode of action and for many more applications. Biogazelle offers a complete solution for all RNA sequencing projects on human, mouse and rat samples supporting basic research, pre-clinical studies and clinical trials.

Related topics: gene expression analysis, mutation detection, fusion gene detection, antisense oligo screening, clinical trials, biomarker discovery, liquid biopsies, non-coding RNA, data analysis

RNA sequencing schematic

“I convinced myself in the first telephone meeting that Biogazelle was at
the leading edge with their technology and science for cancer biology.”

Erik van Beers, VP science and technology at SkylineDx

 

let's start

 

An RNA sequencing workflow for each project

Biogazelle offers a tailored sequencing workflow depending on your project needs. Use the table below to learn which RNA sequencing workflow best fits your application, RNA biotype or sample type. Click the selected workflow name to get access to the related Tech Note.

application

RNA biotype

sample type

workflow

microRNAs and other small RNAs

  • fresh frozen tissue
  • cells
  • fixed tissue
  • whole blood
  • body fluids

small RNA sequencing

protein coding mRNAs

  • fixed tissue
  • body fluids

mRNA capture sequencing

  • fresh frozen tissue
  • cells
  • whole blood
  • stranded polyA+ RNA
  • 3’ end sequencing

protein coding mRNAs and polyadenylated lncRNAs

  • fresh frozen tissue
  • cells
  • whole blood
  • stranded polyA+ RNA
  • 3’ end sequencing

protein coding mRNAs and all long non-coding RNAs

  • fresh frozen tissue
  • cells
  • whole blood

stranded total RNA sequencing

  • fixed tissue
  • body fluids

mRNA + lncRNA capture sequencing

protein coding mRNAs

  • fixed tissue
  • body fluids

mRNA capture sequencing

protein coding mRNAs and polyadenylated lncRNAs

  • fresh frozen tissue
  • cells
  • whole blood

stranded polyA+ RNA sequencing

protein coding mRNAs and all long non-coding RNAs

  • fresh frozen tissue
  • cells
  • whole blood

stranded total RNA sequencing

  • fixed tissue
  • body fluids

mRNA + lncRNA capture sequencing

 

Highlights of our RNA sequencing services

  • including RNA extraction, library preparation, sequencing, data analysis and reporting
  • optimized workflows for formalin-fixed paraffin embedded tissue (FFPE) and liquid biopsies (body fluids, e.g. plasma, serum, urine, cerebrospinal fluid, ...)
  • starting with low amounts of precious patient material (100 ng of cellular RNA, 200 µl of biofluid)
  • tailored sequencing workflows aligned to specific research questions
  • supporting different applications, including a unique and powerful A-Z approach for RNA biomarker discovery and test development
  • scalable IT infrastructure for RNA sequencing data processing of +100 sample projects in a single day, helping you to meet tight timelines
  • comprehensive data analysis capabilities customized to your requirements
  • orthogonal result validation using quantitative / digital PCR technologies
  • optional depletion of unwanted small RNA molecules from the total RNA
  • most comprehensive human reference transcriptome for rich annotation, including Ensembl and LNCipedia
  • performed in an ISO17025 accredited and GCLP-compliant lab