September 18, 2017
Learn from the expert: digital PCR analysis of liquid biopsies
Thanks to recent technological developments, droplet digital PCR (ddPCR) has become an affordable technology for absolute quantification of nucleic acids in biological samples. Droplet digital PCR has several advantages over quantitative PCR as it does not require a calibration curve and is less susceptible to inhibition, providing a more accurate and precise quantification of template DNA molecules and providing a higher resolution to discriminate low frequent sequence variants (e.g. rare mutations in cancer or copy number variant detection).