qbasePLUS manual chapters with accompanying supplemental files
installation and upgrade procedure [PDF]
This chapter guides you through the installation procedure of qbasePLUS. It also describes how you can evaluate the software in fully functional trial mode.
quick guide [PDF]
This quick guide describes how quantitative PCR data can be imported and analyzed in qbasePLUS. It also demonstrates how the built-in quality control can help you to get more reliable results.
whole genome miRNA profiling [PDF]
This chapter describes how to do whole genome miRNA profiling analysis using the global mean normalization strategies implemented in qbasePLUS as of version 2.0 [premium license only].
advanced copy number analysis [PDF]
This chapter describes how you can use qbasePLUS for advanced copy number variant analysis [premium license required].
user interface [PDF]
qbasePLUS is a multi-window application with great flexibility in terms of screen organization. This chapter explains how to work with the qbasePLUS user interface.
data import [PDF]
qbasePLUS has extensive data import functionality. This chapter explains how run data can be directly imported from a wide variety of real-time PCR instruments.
run annotation [PDF]
This chapter explains how well information can be annotated in a PCR run (plate, rotor or chip). In addition, you learn how to create and rename samples & targets and how to copy run layouts.
quality control [PDF]
qbasePLUS provides data quality control on various levels to improve the accuracy and reliability of your results.
PCR efficiency correction [PDF]
PCR efficiency calculation using a standard serial dilution series and efficiency correction in the universal state-of-the-art quantification model employed in qbasePLUS.
genormPLUS [PDF]
qbasePLUS comes with genormPLUS functionality for selection of the best set of reference genes.
inter-run calibration [PDF]
This chapter explains when inter-run calibration is needed and how it is done in qbasePLUS.
sample grouping [PDF]
To enable visual result interpretation and statistical analysis, qbasePLUS 2.0 supports the use of custom sample properties that can be added as extra information for the samples. This chapter describes how custom sample properties can be entered in qbasePLUS and how these can be used.
statistics [PDF]
As of version 2.0, qbasePLUS provides statistical analysis of qPCR results. This chapter explains how to start statistical analysis, describes the results being obtained and provides background information on the available statistical tests.