LncRNA gene expression profiling

lncRNAs represent an emerging important class of regulatory transcribed elements. The study of lncRNAs is timely and relevant, because of their implication in important cellular processes, through new modes of action. The lncRNA genes are generally low abundant, which is exactly why there is a genuine need for a platform with ultimate sensitivity superseding microarray based platforms. Biogazelle’s qPCR service solution is the first to accommodate this need!

Biogazelle has developed a revolutionary platform for accurate and sensitive expression profiling of 1718 human long non-coding RNAs (lncRNAs).

Biogazelle's lncRNA profiling service

lncRNA profiling (1718 human lncRNAs)
250 ng of total RNA
validated high-throughput RT-qPCR workflow using the SmartCycler from Wafergen
data processing using peer-reviewed normalization methods integrated in Biogazelle’s qbase^PLUS software
delivery of high-quality data in a manageable format

Experiments are set-up in 5184-well SmartChips (Wafergen). A low-volume liquid handler enables fast and high-precision pipetting and low volume qPCR reactions to ensure fast turn-around times at affordable costs. The 1718 SYBR Green I RT-qPCR assays are strictly validated according to the MIQE guidelines, and meet Biogazelle’s strict quality control criteria in terms of optimal PCR efficiency, specificity, sensitivity and linear dynamic range of quantification.

Samples reception

Total RNA samples can be shipped to Biogazelle at any time. Customers are strongly advised to follow Biogazelle’s technical guidelines concerning the preparation and delivery of samples. For the entire process 2 ug of total RNA. Good results have been obtained with RNA extracted using the Qiagen miRNeasy kit.

Data processing using qbase^PLUS

Data analysis is performed using our qbasePLUS software, which is built upon a state-of-the-art quantification model. Thorough post-PCR quality control ensures reliable results. The calculation parameters and quality control settings are tailored toward the need of the lincRNA project. Both raw data and processed results (e.g. global mean normalized relative quantities) are delivered in an easy to understand format.

Interesting prices

We are extremely flexible to accommodate your needs and we guarantee exceptional value for money. Please contact our service unit (service@biogazelle.com) or complete the project request form for more information or a quotation. To provide pricing it is convenient to have an idea about the number of samples you would like to analyze.

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