Selected key papers and book chapters on qPCR developments and applications.
If you have an article in which qbasePLUS was used to analyse the data, you can send it to info@biogazelle.com. Each quarter, we will select one contribution and donate a free basic license for qbasePLUS.
2012
Accurate RT-qPCR gene expression analysis on cell culture lysates [PDF]
Scientific Reports.
2012 2:222
keywords: cells-to-ct, cDNA synthesis, cell lysates, high-throughput
2011
Primer Sequence Disclosure: A Clarification of the MIQE Guidelines [PDF]
Clinical Chemistry.
2011 Jun;57(6):919-21
keywords: MIQE
miRNA expression profiling - from reference genes to global mean normalization [PDF]
Methods in Molecular Biology.
822, 261–272 (Humana Press: Totowa, NJ, 2011)
keywords: miRNA profiling, miRNA expression, RT-qPCR, global mean normalization
Whole-genome RT-qPCR microRNA expression profiling[PDF]
Methods in Molecular Biology.
815, 121–130 (Springer New York: New York, NY, 2011)
keywords: miRNA profiling, miRNA expression, RT-qPCR, global mean normalization
Measurable impact of RNA quality on gene expression results from quantitative PCR [PDF]
Nucleic Acids Research.
2011 May;39(9):e63
keywords: RNA quality, gene expression
book chapter Quantitative PCR data analysis – unlocking the secret to successful results
PCR Troubleshooting and Optimization: The Essential Guide
Caister Academic Press 2011, ISBN 978-1-904455-72-1
keywords: data-analysis, relative quantification, statistics, experiment design
2010
The Importance of Quality Control During qPCR Data Analysis [PDF]
International Drug Discovery
August/September 2010
keywords: quality control
How to do successful gene expression analysis using real-time PCR [PDF]
Methods.
2010 Apr;50(4):227-30
keywords: relative quantification, gene expression, workflow
Accurate and objective copy number profiling using real-time quantitative PCR [PDF]
Methods.
2010 Apr;50(4):262-70
keywords: copy number analysis
2009
book chapter Reference gene validation software for improved normalization [PDF]
Real-time PCR: Current Technology and Applications
Academic Press 2009, ISBN 978-1-904455-39-4
keywords: validation of reference genes (formerly housekeeping genes)
RTPrimerDB: the portal for real-time PCR primers and probes [PDF]
Nucleic Acids Res. 2009 Jan;37(Database issue):D942-5
keywords: qPCR assay database, in silico assay quality control
RDML: structured language and reporting guidelines for real-time quantitative PCR data [PDF]
Nucleic Acids Res. 2009 Apr;37(7):2065-9
keywords: RDML, qPCR data standard
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments [PDF]
Clin Chem. 2009 Apr;55(4):611-22
keywords: MIQE, reporting guidelines
External oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR data [PDF]
Nucleic Acids Res. 2009 Nov;37(21):e138
keywords: collaboration, inter-run calibration
RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts [PDF]
BMC Res Notes. 2009 Nov 25;2:235
keywords: RNA pre-amplification
A novel and universal method for microRNA RT-qPCR data normalization [PDF]
Genome Biol. 2009;10(6):R64
keywords: miRNA, normalization
2008
Standardization of real-time PCR gene expression data from independent biological replicates [PDF]
Anal Biochem. 2008 Aug 1;379(1):127-9
keywords: data standardization, statistics
High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA [PDF]
Nucleic Acids Res. 2008 Dec;36(21):e143
keywords: miRNA expression, pre-amplification
2007
qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data [PDF]
Genome Biol. 2007;8(2):R19
keywords: mathematical framework, experiment design
2006
book chapter Real-time quantitative PCR as an alternative to Southern blot or FISH for detection of gene copy number changes [PDF]
Protocols for Nucleic Acid Analysis with Non-Radioactive Probes
Humana Press 2006, ISBN 1-58829-430-7
keywords: copy number analysis
RTPrimerDB: the real-time PCR primer and probe database, major update 2006 [PDF]
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D684-8
keywords: qPCR assay database, in silico assay quality control
Real-time quantitative allele discrimination assay using 3' LNA primers for detection of low-percentage mosaic mutations [PDF]
Anal Biochem. 2006 Dec 1;359(1):144-6
keywords: mutation analysis
2005
Rapid detection of VHL exon deletions using real-time quantitative PCR [PDF]
Lab Invest. 2005 Jan;85(1):24-33
keywords: copy number analysis
Impact of RNA quality on reference gene expression stability [PDF]
Biotechniques. 2005 Jul;39(1):52, 54, 56
keywords: RNA quality, reference gene stability
2002
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes [PDF]
Genome Biol. 2002 Jun 18;3(7):RESEARCH0034
keywords: geNorm, multi-gene normalization
Elimination of primer-dimer artefacts and genomic co-amplification using a two-step SYBR Green I real-time RT-PCR [PDF]
Anal Biochem. 2002 Apr 1;303(1):95-8
keywords: two-step RT-qPCR, elimination of primer dimers
2001
Quantification of splice variants using real-time PCR [PDF]
Nucleic Acids Res. 2001 Jul 1;29(13):E68-8
keywords: splice variants