Selected key papers and book chapters on qPCR developments and applications.
2010
book chapter qPCR data analysis – unlocking the secret to successful results
PCR Troubleshooting and Optimization: The Essential Guide
Horizon Press 2010
keywords: data-analysis, relative quantification, statistics, experiment design
How to do successful gene expression analysis using real-time PCR [PDF]
Methods (in press)
keywords: relative quantification, gene expression, workflow
Accurate and objective copy number profiling using real-time quantitative PCR [PDF]
Methods (in press)
keywords: copy number analysis
2009
book chapter Reference gene validation software for improved normalization [PDF]
Real-time PCR: Current Technology and Applications
Academic Press 2009, ISBN 978-1-904455-39-4
keywords: validation of reference genes (formerly housekeeping genes)
RTPrimerDB: the portal for real-time PCR primers and probes [PDF]
Nucleic Acids Res. 2009 Jan;37(Database issue):D942-5
keywords: qPCR assay database, in silico assay quality control
RDML: structured language and reporting guidelines for real-time quantitative PCR data [PDF]
Nucleic Acids Res. 2009 Apr;37(7):2065-9
keywords: RDML, qPCR data standard
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments [PDF]
Clin Chem. 2009 Apr;55(4):611-22
keywords: MIQE, reporting guidelines
External oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR data [PDF]
Nucleic Acids Res. 2009 Nov;37(21):e138
keywords: collaboration, inter-run calibration
RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts [PDF]
BMC Res Notes. 2009 Nov 25;2:235
keywords: RNA pre-amplification
A novel and universal method for microRNA RT-qPCR data normalization [PDF]
Genome Biol. 2009;10(6):R64
keywords: miRNA, normalization
2008
Standardization of real-time PCR gene expression data from independent biological replicates [PDF]
Anal Biochem. 2008 Aug 1;379(1):127-9
keywords: data standardization, statistics
High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA [PDF]
Nucleic Acids Res. 2008 Dec;36(21):e143
keywords: miRNA expression, pre-amplification
2007
qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data [PDF]
Genome Biol. 2007;8(2):R19
keywords: mathematical framework, experiment design
2006
book chapter Real-time quantitative PCR as an alternative to Southern blot or FISH for detection of gene copy number changes [PDF]
Protocols for Nucleic Acid Analysis with Non-Radioactive Probes
Humana Press 2006, ISBN 1-58829-430-7
keywords: copy number analysis
RTPrimerDB: the real-time PCR primer and probe database, major update 2006 [PDF]
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D684-8
keywords: qPCR assay database, in silico assay quality control
Real-time quantitative allele discrimination assay using 3' LNA primers for detection of low-percentage mosaic mutations [PDF]
Anal Biochem. 2006 Dec 1;359(1):144-6
keywords: mutation analysis
2005
Rapid detection of VHL exon deletions using real-time quantitative PCR [PDF]
Lab Invest. 2005 Jan;85(1):24-33
keywords: copy number analysis
Impact of RNA quality on reference gene expression stability [PDF]
Biotechniques. 2005 Jul;39(1):52, 54, 56
keywords: RNA quality, reference gene stability
2002
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes [PDF]
Genome Biol. 2002 Jun 18;3(7):RESEARCH0034
keywords: geNorm, multi-gene normalization
Elimination of primer-dimer artefacts and genomic co-amplification using a two-step SYBR Green I real-time RT-PCR [PDF]
Anal Biochem. 2002 Apr 1;303(1):95-8
keywords: two-step RT-qPCR, elimination of primer dimers
2001
Quantification of splice variants using real-time PCR [PDF]
Nucleic Acids Res. 2001 Jul 1;29(13):E68-8
keywords: splice variants