genormPLUS accelerates your quest for optimal reference genes in many ways
geNorm is the most popular algorithm to find the stable reference (housekeeping) genes from a set of tested candidate reference genes in a given experimental condition. From this, a gene expression normalization factor can be calculated for each sample, based on the geometric mean of a user-defined number of reference genes.
Integration of geNorm in the qbasePLUS/qPCR data-analysis software provides you fully automated calculations, handling of missing data, ranking of candidate reference genes up to the single most stable gene and expert reporting for in depth understanding of your geNorm pilot study. And all of this can be done on your operating system of choice (Windows, Mac, Linux).
Benefits of the genormPLUS in comparison to geNorm
- Fully automated calculations (input contains Cq values)
Calculation of efficiency corrected relative quantities and geNorm analysis are combined in a single program to speed up analysis. Previously, many manual calculations were needed in Excel.
- Handles missing data
Experiments with missing data are automatically processed in a way that has the lowest impact on the overall analysis through intelligent retention of as many data points as possible.
- Expert report
Expert report with recommendations on the number and identity of genes to be used for optimal normalization as well as information on the relative suitability of the selected genes.
- Platform independent
qbasePLUS and its genormPLUS implementation run on Windows, Mac, Linux.
- Reference gene ranking
Whereas geNorm Excel could not distinguish the two most stably expressed genes, the modified algorithm in genormPLUS allows ranking of candidate reference genes up to the single most stable gene.