Experiment Name,RQ example
Run Start,8/20/2010 10:50:29 PM
Run Finish,8/20/2010 11:33:28 PM
Application,Quantification/Hydrolysis Probe/DNA/Relative Quantification
Instrument,Eco™ Real-Time PCR System
Software,Eco™ Software
Last Modified,11/8/2010 7:21:45 PM

RQ Analysis Method:,Singleplex
Reference Assay:,B2M,
Reference Sample:,pooled

Assay Name,PCR Efficiency %
B2M,100.000
TRa,100.000

Well,Exclude,Sample Name,Assay Name,Assay Role,Cq,Cq Mean,Std. Dev. Cq,ΔCq,ΔCq Mean,Std. Dev. ΔCq,ΔΔCq,Relative Expression,Expression Min,Expression Max,Threshold Setting,Cq Threshold,Baseline Setting,Baseline Start,Baseline End,Tm1,Tm2,Tm3,
A1,,pooled,B2M,Unknown,23.2520,23.2268594,0.0356080,,,,,,,,Manual,0.0206207,Manual,3,15,,,,
A2,,pooled,B2M,Unknown,23.2017,23.2268594,0.0356080,,,,,,,,Manual,0.0206207,Manual,3,15,,,,
A3,,pooled,TRa,Unknown,28.2168,28.1127457,0.1471777,,4.8858863,0.1514240,0.0000000,1.0000000,0.9003613,1.1106652,Manual,0.0326782,Manual,3,15,,,,
A4,,pooled,TRa,Unknown,28.0087,28.1127457,0.1471777,,4.8858863,0.1514240,0.0000000,1.0000000,0.9003613,1.1106652,Manual,0.0326782,Manual,3,15,,,,
A5,
A6,
A7,
A8,
B1,,heart,B2M,Unknown,24.1813,24.1812639,,,,,,,,,Manual,0.0206207,Manual,3,15,,,,
B2,Excluded,heart,B2M,Unknown,,,,,,,,,,,Manual,0.0206207,Manual,3,15,,,,
B3,,heart,TRa,Unknown,28.5018,28.5907991,0.1259071,,4.4095352,,-0.4763511,1.3912205,,,Manual,0.0326782,Manual,3,15,,,,
B4,,heart,TRa,Unknown,28.6798,28.5907991,0.1259071,,4.4095352,,-0.4763511,1.3912205,,,Manual,0.0326782,Manual,3,15,,,,
B5,
B6,
B7,
B8,
C1,,kidney,B2M,Unknown,22.4690,22.5148941,0.0648763,,,,,,,,Manual,0.0206207,Manual,3,15,,,,
C2,,kidney,B2M,Unknown,22.5608,22.5148941,0.0648763,,,,,,,,Manual,0.0206207,Manual,3,15,,,,
C3,,kidney,TRa,Unknown,27.8652,27.8234409,0.0591152,,5.3085468,0.0877698,0.4226605,0.7460475,0.7020130,0.7928442,Manual,0.0326782,Manual,3,15,,,,
C4,,kidney,TRa,Unknown,27.7816,27.8234409,0.0591152,,5.3085468,0.0877698,0.4226605,0.7460475,0.7020130,0.7928442,Manual,0.0326782,Manual,3,15,,,,
C5,
C6,
C7,
C8,
D1,,liver,B2M,Unknown,23.2651,23.3491399,0.1188933,,,,,,,,Manual,0.0206207,Manual,3,15,,,,
D2,,liver,B2M,Unknown,23.4332,23.3491399,0.1188933,,,,,,,,Manual,0.0206207,Manual,3,15,,,,
D3,,liver,TRa,Unknown,31.5337,31.4350937,0.1394594,,8.0859538,0.1832609,3.2000675,0.1088137,0.0958333,0.1235523,Manual,0.0326782,Manual,3,15,,,,
D4,,liver,TRa,Unknown,31.3365,31.4350937,0.1394594,,8.0859538,0.1832609,3.2000675,0.1088137,0.0958333,0.1235523,Manual,0.0326782,Manual,3,15,,,,
D5,
D6,
D7,
D8,
E1,,lung,B2M,Unknown,22.5032,22.4592868,0.0620582,,,,,,,,Manual,0.0206207,Manual,3,15,,,,
E2,,lung,B2M,Unknown,22.4154,22.4592868,0.0620582,,,,,,,,Manual,0.0206207,Manual,3,15,,,,
E3,,lung,TRa,Unknown,29.2536,29.2430982,0.0148643,,6.7838114,0.0638135,1.8979252,0.2683290,0.2567189,0.2804642,Manual,0.0326782,Manual,3,15,,,,
E4,,lung,TRa,Unknown,29.2326,29.2430982,0.0148643,,6.7838114,0.0638135,1.8979252,0.2683290,0.2567189,0.2804642,Manual,0.0326782,Manual,3,15,,,,
E5,
E6,
E7,
E8,
F1,,,B2M,Unknown,,,,,,,,,,,Manual,0.0206207,Manual,3,15,,,,
F2,,,B2M,Unknown,,,,,,,,,,,Manual,0.0206207,Manual,3,15,,,,
F3,,,TRa,NTC,,,,,,,,,,,Manual,0.0326782,Manual,3,15,,,,
F4,,,TRa,NTC,,,,,,,,,,,Manual,0.0326782,Manual,3,15,,,,
F5,
F6,
F7,
F8,