Experiment Name,Stendal1_biogazelle
Run Start,3/23/2011 10:38:34 AM
Run Finish,3/23/2011 1:08:47 PM
Application,Quantification/Hydrolysis Probe/DNA/Standard Curve
Instrument,Eco™ Real-Time PCR System
Software,Eco™ Software v3.0.16.0
Last Modified,5/5/2011 10:43:14 PM

Standard Curve Data
Assay Name,Slope,Intercept,R2,Efficiency %,
Chlamydia,-3.4100384,47.5263733,0.9984943,96.4499060,
Neospora,-3.7502397,46.3840858,0.9745958,84.7777290,
Coxiella,-4.9270296,57.8147409,0.8842647,59.5739674,

Well,Exclude,Sample Name,Assay Name,Assay Role,Cq,Cq Mean,Std. Dev. Cq,Quantity,Quantity Unit,Mean Qty.,Std. Dev. Qty.,Threshold Setting,Cq Threshold,Baseline Setting,Baseline Start,Baseline End,Tm1,Tm2,Tm3,
A1,,Wasser,Chlamydia,Unknown,38.4674145,40.0964696,2.2769929,453.4780,copies,256.8877811,215.7606359,Manual,0.0080000,Manual,6,10,,,,
A1,,Wasser,Neospora,Unknown,39.0166498,38.6055371,0.5814012,92.1561,copies,122.4159348,42.7938809,Manual,0.0050000,Manual,6,10,,,,
A1,,Wasser,Coxiella,Unknown,,,,,,,,Manual,0.0110000,Manual,6,10,,,,
A1,,Wasser,Beta-Actin,Unknown,37.0180447,37.4987377,0.4170713,,,,,Manual,0.0090000,Manual,6,10,,,,
A2,,chlamydian plasmid,Chlamydia,Standard,17.0209427,17.0209427,,1000000000.0000,copies,1000000000.0000000,0.0000000,Manual,0.0080000,Manual,6,10,,,,
A2,,chlamydian plasmid,Neospora,Unknown,,34.3232294,,,,1644.4581947,,Manual,0.0050000,Manual,6,10,,,,
A2,,chlamydian plasmid,Coxiella,Unknown,,,,,,,,Manual,0.0110000,Manual,6,10,,,,
A2,,chlamydian plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
A3,,chlamydian plasmid,Chlamydia,Standard,20.1777043,20.1777043,,100000000.0000,copies,100000000.0000000,0.0000000,Manual,0.0080000,Manual,6,10,,,,
A3,,chlamydian plasmid,Neospora,Unknown,,34.3232294,,,,1644.4581947,,Manual,0.0050000,Manual,6,10,,,,
A3,,chlamydian plasmid,Coxiella,Unknown,,,,,,,,Manual,0.0110000,Manual,6,10,,,,
A3,,chlamydian plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
A4,,chlamydian plasmid,Chlamydia,Standard,23.4944436,23.4944436,,10000000.0000,copies,10000000.0000000,0.0000000,Manual,0.0080000,Manual,6,10,,,,
A4,,chlamydian plasmid,Neospora,Unknown,,34.3232294,,,,1644.4581947,,Manual,0.0050000,Manual,6,10,,,,
A4,,chlamydian plasmid,Coxiella,Unknown,,,,,,,,Manual,0.0110000,Manual,6,10,,,,
A4,,chlamydian plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
A5,,chlamydian plasmid,Chlamydia,Standard,26.8548863,26.8548863,,1000000.0000,copies,1000000.0000000,0.0000000,Manual,0.0080000,Manual,6,10,,,,
A5,,chlamydian plasmid,Neospora,Unknown,,34.3232294,,,,1644.4581947,,Manual,0.0050000,Manual,6,10,,,,
A5,,chlamydian plasmid,Coxiella,Unknown,,,,,,,,Manual,0.0110000,Manual,6,10,,,,
A5,,chlamydian plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
A6,,chlamydian plasmid,Chlamydia,Standard,30.7325439,30.7325439,,100000.0000,copies,100000.0000000,0.0000000,Manual,0.0080000,Manual,6,10,,,,
A6,,chlamydian plasmid,Neospora,Unknown,,34.3232294,,,,1644.4581947,,Manual,0.0050000,Manual,6,10,,,,
A6,,chlamydian plasmid,Coxiella,Unknown,,,,,,,,Manual,0.0110000,Manual,6,10,,,,
A6,,chlamydian plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
A7,,chlamydian plasmid,Chlamydia,Standard,,,,10000.0000,copies,10000.0000000,0.0000000,Manual,0.0080000,Manual,6,10,,,,
A7,,chlamydian plasmid,Neospora,Unknown,,34.3232294,,,,1644.4581947,,Manual,0.0050000,Manual,6,10,,,,
A7,,chlamydian plasmid,Coxiella,Unknown,,,,,,,,Manual,0.0110000,Manual,6,10,,,,
A7,,chlamydian plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
A8,,chlamydian plasmid,Chlamydia,Standard,,,,1000.0000,copies,1000.0000000,0.0000000,Manual,0.0080000,Manual,6,10,,,,
A8,,chlamydian plasmid,Neospora,Unknown,34.3232294,34.3232294,,1644.4582,copies,1644.4581947,,Manual,0.0050000,Manual,6,10,,,,
A8,,chlamydian plasmid,Coxiella,Unknown,,,,,,,,Manual,0.0110000,Manual,6,10,,,,
A8,,chlamydian plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
B1,,chlamydian plasmid,Chlamydia,Standard,,,,100.0000,copies,100.0000000,0.0000000,Manual,0.0080000,Manual,6,10,,,,
B1,,chlamydian plasmid,Neospora,Unknown,,34.3232294,,,,1644.4581947,,Manual,0.0050000,Manual,6,10,,,,
B1,,chlamydian plasmid,Coxiella,Unknown,,,,,,,,Manual,0.0110000,Manual,6,10,,,,
B1,,chlamydian plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
B2,
B3,
B4,
B5,
B6,
B7,
B8,
C1,,Wasser,Chlamydia,Unknown,42.6982663,40.0964696,2.2769929,26.0526,copies,256.8877811,215.7606359,Manual,0.0080000,Manual,6,10,,,,
C1,,Wasser,Neospora,Unknown,38.1944244,38.6055371,0.5814012,152.6758,copies,122.4159348,42.7938809,Manual,0.0050000,Manual,6,10,,,,
C1,,Wasser,Coxiella,Unknown,,,,,,,,Manual,0.0110000,Manual,6,10,,,,
C1,,Wasser,Beta-Actin,Unknown,37.7136051,37.4987377,0.4170713,,,,,Manual,0.0090000,Manual,6,10,,,,
C2,,neospora plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
C2,,neospora plasmid,Neospora,Standard,13.1269823,13.1269823,,1000000000.0000,copies,1000000000.0000000,0.0000000,Manual,0.0050000,Manual,6,10,,,,
C2,,neospora plasmid,Coxiella,Unknown,41.2365402,37.4210646,3.3662357,2316.0369,copies,29224.4944928,33836.2071495,Manual,0.0110000,Manual,6,10,,,,
C2,,neospora plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
C3,,neospora plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
C3,,neospora plasmid,Neospora,Standard,16.9811043,16.9811043,,100000000.0000,copies,100000000.0000000,0.0000000,Manual,0.0050000,Manual,6,10,,,,
C3,,neospora plasmid,Coxiella,Unknown,35.5930762,37.4210646,3.3662357,32370.8766,copies,29224.4944928,33836.2071495,Manual,0.0110000,Manual,6,10,,,,
C3,,neospora plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
C4,,neospora plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
C4,,neospora plasmid,Neospora,Standard,20.3476594,20.3476594,,10000000.0000,copies,10000000.0000000,0.0000000,Manual,0.0050000,Manual,6,10,,,,
C4,,neospora plasmid,Coxiella,Unknown,33.7702380,37.4210646,3.3662357,75878.9691,copies,29224.4944928,33836.2071495,Manual,0.0110000,Manual,6,10,,,,
C4,,neospora plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
C5,,neospora plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
C5,,neospora plasmid,Neospora,Standard,23.9858113,23.9858113,,1000000.0000,copies,1000000.0000000,0.0000000,Manual,0.0050000,Manual,6,10,,,,
C5,,neospora plasmid,Coxiella,Unknown,39.0844039,37.4210646,3.3662357,6332.0954,copies,29224.4944928,33836.2071495,Manual,0.0110000,Manual,6,10,,,,
C5,,neospora plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
C6,,neospora plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
C6,,neospora plasmid,Neospora,Standard,26.3533813,26.3533813,,100000.0000,copies,100000.0000000,0.0000000,Manual,0.0050000,Manual,6,10,,,,
C6,,neospora plasmid,Coxiella,Unknown,,37.4210646,3.3662357,,,29224.4944928,33836.2071495,Manual,0.0110000,Manual,6,10,,,,
C6,,neospora plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
C7,,neospora plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
C7,,neospora plasmid,Neospora,Standard,29.0284371,29.0284371,,10000.0000,copies,10000.0000000,0.0000000,Manual,0.0050000,Manual,6,10,,,,
C7,,neospora plasmid,Coxiella,Unknown,,37.4210646,3.3662357,,,29224.4944928,33836.2071495,Manual,0.0110000,Manual,6,10,,,,
C7,,neospora plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
C8,,neospora plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
C8,,neospora plasmid,Neospora,Standard,,,,1000.0000,copies,1000.0000000,0.0000000,Manual,0.0050000,Manual,6,10,,,,
C8,,neospora plasmid,Coxiella,Unknown,,37.4210646,3.3662357,,,29224.4944928,33836.2071495,Manual,0.0110000,Manual,6,10,,,,
C8,,neospora plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
D1,,neospora plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
D1,,neospora plasmid,Neospora,Standard,41.1053988,41.1053988,,100.0000,copies,100.0000000,0.0000000,Manual,0.0050000,Manual,6,10,,,,
D1,,neospora plasmid,Coxiella,Unknown,,37.4210646,3.3662357,,,29224.4944928,33836.2071495,Manual,0.0110000,Manual,6,10,,,,
D1,,neospora plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
D2,
D3,
D4,
D5,
D6,
D7,
D8,
E1,,Wasser,Chlamydia,Unknown,39.1237280,40.0964696,2.2769929,291.1328,copies,256.8877811,215.7606359,Manual,0.0080000,Manual,6,10,,,,
E1,,Wasser,Neospora,Unknown,,38.6055371,0.5814012,,,122.4159348,42.7938809,Manual,0.0050000,Manual,6,10,,,,
E1,,Wasser,Coxiella,Unknown,,,,,,,,Manual,0.0110000,Manual,6,10,,,,
E1,,Wasser,Beta-Actin,Unknown,37.7645632,37.4987377,0.4170713,,,,,Manual,0.0090000,Manual,6,10,,,,
E2,,coxiella plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
E2,,coxiella plasmid,Neospora,Unknown,,39.6894003,2.6090700,,,104.4547273,119.9426605,Manual,0.0050000,Manual,6,10,,,,
E2,,coxiella plasmid,Coxiella,Standard,15.3913565,15.3913565,,1000000000.0000,copies,1000000000.0000000,0.0000000,Manual,0.0110000,Manual,6,10,,,,
E2,,coxiella plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
E3,,coxiella plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
E3,,coxiella plasmid,Neospora,Unknown,,39.6894003,2.6090700,,,104.4547273,119.9426605,Manual,0.0050000,Manual,6,10,,,,
E3,,coxiella plasmid,Coxiella,Standard,18.8134752,18.8134752,,100000000.0000,copies,100000000.0000000,0.0000000,Manual,0.0110000,Manual,6,10,,,,
E3,,coxiella plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
E4,,coxiella plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
E4,,coxiella plasmid,Neospora,Unknown,,39.6894003,2.6090700,,,104.4547273,119.9426605,Manual,0.0050000,Manual,6,10,,,,
E4,,coxiella plasmid,Coxiella,Standard,22.6147766,22.6147766,,10000000.0000,copies,10000000.0000000,0.0000000,Manual,0.0110000,Manual,6,10,,,,
E4,,coxiella plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
E5,,coxiella plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
E5,,coxiella plasmid,Neospora,Unknown,,39.6894003,2.6090700,,,104.4547273,119.9426605,Manual,0.0050000,Manual,6,10,,,,
E5,,coxiella plasmid,Coxiella,Standard,25.8838190,25.8838190,,1000000.0000,copies,1000000.0000000,0.0000000,Manual,0.0110000,Manual,6,10,,,,
E5,,coxiella plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
E6,,coxiella plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
E6,,coxiella plasmid,Neospora,Unknown,,39.6894003,2.6090700,,,104.4547273,119.9426605,Manual,0.0050000,Manual,6,10,,,,
E6,,coxiella plasmid,Coxiella,Standard,28.7900537,28.7900537,,100000.0000,copies,100000.0000000,0.0000000,Manual,0.0110000,Manual,6,10,,,,
E6,,coxiella plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
E7,,coxiella plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
E7,,coxiella plasmid,Neospora,Unknown,,39.6894003,2.6090700,,,104.4547273,119.9426605,Manual,0.0050000,Manual,6,10,,,,
E7,,coxiella plasmid,Coxiella,Standard,43.2408084,43.2408084,,10000.0000,copies,10000.0000000,0.0000000,Manual,0.0110000,Manual,6,10,,,,
E7,,coxiella plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
E8,,coxiella plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
E8,,coxiella plasmid,Neospora,Unknown,41.5342914,39.6894003,2.6090700,19.6425,copies,104.4547273,119.9426605,Manual,0.0050000,Manual,6,10,,,,
E8,,coxiella plasmid,Coxiella,Standard,,,,1000.0000,copies,1000.0000000,0.0000000,Manual,0.0110000,Manual,6,10,,,,
E8,,coxiella plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
F1,,coxiella plasmid,Chlamydia,Unknown,,,,,,,,Manual,0.0080000,Manual,6,10,,,,
F1,,coxiella plasmid,Neospora,Unknown,37.8445093,39.6894003,2.6090700,189.2670,copies,104.4547273,119.9426605,Manual,0.0050000,Manual,6,10,,,,
F1,,coxiella plasmid,Coxiella,Standard,,,,100.0000,copies,100.0000000,0.0000000,Manual,0.0110000,Manual,6,10,,,,
F1,,coxiella plasmid,Beta-Actin,Unknown,,,,,,,,Manual,0.0090000,Manual,6,10,,,,
F2,
F3,
F4,
F5,
F6,
F7,
F8,